Volume 8
Number 1 January 2024Isolation, identification and antibiotic sensitivity patterns of bacteria in Early onset and Late onset neonatal 08 septicemia at a tertiary care hospital
DOI: https://doi.org/10.70357/jdamc.2024.v0801.03
Afrin M1 , Showkath MS2 , Parvin S3 , Sharmin R4 , Biswas MA5 , Ahommed F6 , Sarker A7
Abstract
This is a descriptive type of cross sectional study which was done to find out the causative organisms and their antibiotic sensitivities in the Department of Microbiology of Rajshahi Medical College Hospital, Rajshahi during the period of July 2014 to June 2015. A total of 116 blood samples were taken aseptically from the suspected neonatal septicemic patients which were then inoculated in Brain heart infusion broth. Isolation, identification and antibiotic sensitivity pattern of Bacteria were done by standard microbiological methods. 33(28.4%) cases were found to be culture positive among 116 cases. Among 33 culture positive cases EONS was 19 (57.57%) and LONS was 14 (42.42%). The isolated causative organisms of EONS were 10 (52.6%) by Staphylococcus aureus, 1 (5.3%) by Streptococcus pneumoniae, 7 (36.8%) by E.coli and 1 (5.3%) by Klebsiella pneumoniae. On the other hand, causative agents of LONS were 7(50%) by Staphylococcus aureus, 2 (14.3%) by Streptococcus pneumoniae, 3 (21.4%) by E.coli, 1 (7.1%) by Klebsiella pneumoniae and 1 (7.1%) by Pseudomonas aeruginosa. All the cases of Gram positive and Gram negative organisms were highly sensitive to meropenem, amikacin, gentamicin and ciprofloxacin. Gram positive bacteria were found to be particularly sensitive to vancomycin. They were moderately sensitive to ceftazidime, amoxicillin but were completely resistant to ampicillin. This study revealed the predominance of Staphylococcus aureus and E.coli as causative organisms in both EONS and LONS and these organisms are markedly sensitive to meropenem, amikacin, gentamicin and ciprofloxacin.
Keywords: Early onset neonatal septicemia (EONS), late onset neonatal septicemia (LONS), causative agents, antibiotic sensitivity pattern.
- Associate Professor, Head of the Department of Microbiology
- Associate Professor, Department of Microbiology
- Assistant Professor, Department of Pathology
- Associate Professor, Department of Microbiology
- Associate Professor, Department of Respiratory Medicine
- Senior Consultant, Department of Cardiology
- Medical Officer, Department of Microbiology